I-Porcine Acute Diarrhea Syndrome Coronavirus (SADS-CoV) iyi-porcine enteric pathogenic coronavirus esanda kutholakala engabangela uhudo olumanzi emaphuphuneni asanda kuzalwa futhi ibangele ukulahlekelwa okukhulu kwezomnotho embonini yezingulube.Njengamanje, ayikho indlela efanelekile ye-serological yokuhlola ukusebenza kahle kokutheleleka kwe-SADS-CoV kanye nomuthi wokugoma, ngakho kunesidingo esiphuthumayo sokusebenzisa i-enzyme-linked immunosorbent assay (ELISA) esebenzayo yokwenza lokhu kuntuleka.Lapha, i-plasmid recombinant eveza i-SADS-CoV spike (S) protein ehlanganiswe nesizinda somuntu se-IgG Fc yakhiwe ukuze kukhiqizwe i-baculovirus ephindayo futhi yavezwa kumaseli e-HEK 293F.Iphrotheni ye-S-Fc ihlanzwa nge-protein G resin futhi igcina ukusebenza kabusha ngama-anti-human Fc kanye nama-anti-SADS-CoV amasosha omzimba.Khona-ke amaprotheni e-S-Fc asetshenziselwa ukuthuthukisa i-ELISA engaqondile (S-iELISA) nokwandisa izimo zokusabela ze-S-iELISA.Njengomphumela, ngokuhlaziya inani le-OD450nm lama-40 SADS-CoV angenayo isera eliqinisekiswe i-immunofluorescence assay (IFA) kanye ne-Western blotting, inani elinqunyiwe lanqunywa ukuthi libe ngu-0.3711.I-coefficient of variation (CV) ye-6 SADS-CoV positive sera ngaphakathi naphakathi kokugijima kwe-S-iELISA konke bekungaphansi kuka-10%.Ukuhlolwa kwe-cross-reactivity kubonise ukuthi i-S-iELISA ayinakho ukuhlangana kabusha nenye i-sera ye-porcine virus.Ukwengeza, ngokusekelwe ekutholeni amasampula e-serum yomtholampilo we-111, izinga lokuhlangana kwe-IFA kanye ne-S-iELISA lalingama-97.3%.Ukuhlolwa kokungathathi hlangothi kwegciwane okunamavelu angu-7 ahlukene e-OD450nm ye-serum kubonise ukuthi inani le-OD450nm elitholwe yi-S-iELISA lalihlotshaniswa kahle nokuhlolwa kwe-virus neutralization.Ekugcineni, i-S-iELISA yenziwa kumasampula e-serum epulazi lezingulube angama-300.Amakhithi okuhweba amanye ama-porcine enteroviruses abonise ukuthi amanani e-IgG positive we-SADS-CoV, TGEV, PDCoV kanye ne-PEDV ayengu-81.7%, 54%, kanye no-65.3%, ngokulandelana., 6%, ngokulandelana.Imiphumela ibonisa ukuthi i-S-iELISA icacile, iyazwela, futhi iyakwazi ukukhiqizwa, futhi ingasetshenziswa ukuthola ukutheleleka kwe-SADS-CoV embonini yezingulube.Lesi sihloko sivikelwe yi-copyright.wonke Amalungelo Agodliwe.
Isikhathi sokuthumela: Jun-22-2021